The OV-1 served in the U.S. Army in Vietnam and during Operation Desert Storm.

Starting in 1972, the Army National Guard (ARNG) began to receive the Mohawk, with the ARNG eventually operating 13 OV-1Bs, 24 OV-1Cs, and 16 OV-1Ds serving with three aviation units in Georgia and Oregon.

U.S. Army OV-1s were retired from Europe in 1992, from Korea in September 1996, and finally in the US in 1996, superseded by newer systems, newer aircraft, and the evolution of spy satellites. The OV-1 was primarily replaced by militarized version of the de Havilland DH-7 turboprop commuter airliner equipped with a SLAR system until the U.S. Air Force's E-8 J-STARS (Joint Surveillance Target Attack Radar System) aircraft, based on converted Boeing 707 airframes with powerful side-looking radar, became fully operational.

As of 2011, Alliant Techsystems has partnered with the Broadbay Group and Mohawk Technologies of Florida in a venture to return an armed, modernized version of the OV-1D to operational use as a counter-insurgency aircraft. A demonstrator has been equipped with a FLIR Star Safire turret and a ventral, trainable M230 Chain Gun.